Guanadine HCL is one of the strongest denaturing agents for protein folding studies, and is used to denature proteins due to its chaotropic qualitiesSafety Data Sheet Guanidine Hydrochloride Section 1 Identification 11 Product Identifiers Product Name Guanidine Hydrochloride CAS# RTECS# MF Synonyms Guanidine Monohydrochloride, Guanidinium Chloride, Guanidinium Hydrochloride 12 Recommended Use of the Chemical and restrictions of Use Chemical manufacturing 13 SupplierDirect addition of SDS to samples containing guanidine·HCl results in the formation of the insoluble guanidinium salt of dodecyl sulfate The guanidine·HCl must therefore be removed before addition of the sample buffer used for SDSPAGE analysis ( UNIT 101 )
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Guanidine hcl sds-SDS information may be continued on back of page Safety Data Sheet Revision Isopropanol SDS and Guanidine Hydrochloride Guanidine Hydrochloride, Sodium Hydroxide and Isopropanol Hazard information is provided for compliance with both the UK Chemicals (Hazard Information and Packaging) (CHIP) Regulations and the US HazardMost proteins will not renature, but precipitate if you remove or dilute guanidine hydrochloride Thus, you get a precipitate that you can wash with water and dissolve in
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Guanidine hydrochloride oral 556,5 mg/kg Guanidine hydrochloride inhalation dust/mist 3,181 mg/l/4h Skin corrosion/irritation Causes skin irritation Serious eye damage/eye irritation Causes serious eye irritation Respiratory or skin sensitisation Shall not be classified as a respiratory or skin sensitiser Safety data sheetThe activity of TEV Protease on an MBPfusion protein in the presence of these denaturants has been reported by C Sun et al as follows Urea In up to 2 M urea near complete cleavage is detectedThere is some inhibition of TEV Protease cleavage in urea concentrations of 4 M Guanidine HClGuanidinium chloride Recommended Use Laboratory chemicals Uses advised against Not for food, drug, pesticide or
H319 Causes serious eye irritation Precautionary Statements P501 Dispose of contents/ container to an approved waste disposal plant P270 Do not eat, drink or smoke when using this product P264 Wash skin thoroughly after handling P280 Wear protective gloves/ eye protection/ face protectionSynonyms Aminoformamidine hydrochloride,Aminomethanamidine hydrochloride,Guanidinium chloride;Guanidine Hydrochloride 6M Solution Safety Data Sheet According to Regulation (EC) 1272/08 Issue Date Revision date SECTION 1 Identification 11 Product Identifiers Product Name Guanidine Hydrochloride 6M Solution CAS # EC #
Structure Guanidinium chloride crystallizes in orthorhombic space group PbcaThe crystal structure consists of a network of guanidinium cations and chloride anions linked by N–H···Cl hydrogen bonds Acidity Guanidinium chloride is a weak acid with a pK a of 136 The reason that it is such a weak acid is the complete delocalisation of the positive charge through 3 nitrogen atoms (plusSAFETY DATA SHEET Lysis and Binding Buffer (LBB) Page 1 of 5 Bionano Genomics, Inc SDS375 Rev A 1 CHEMICAL PRODUCT AND COMPANY IDENTIFICATION Guanidine hydrochlorideTris (25 mM), Glycine (192 M), SDS (01%), pH , undiluted, no interference Zinc chloride (10 mM) in TBS, pH 72, 10 mM Buffer Additives Ammonium sulfate 15 mM Aprotinin 10 mg/L Cesium bicarbonate 100 mM Glucose 10 mM Glycerol 10% Guanidine•HCl 4 M Hydrochloric acid 100 mM Imidazole 50 mM Leupeptin 10 mg/L PMSF 1 mM Sodium azide 0%
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Guanidine Hydrochloride, Molecular Biology Grade CAS Calbiochem Guanidine Hydrochloride, Molecular Biology Grade CAS Calbiochem Customer Support Contact Us FAQ Safety Data Sheets (SDS) Certificates (COA/COO) Quality & Regulatory Calculators & Apps Webinars Orders Quick Order Custom Products Commerce SolutionsSafety Data Sheet according to WHS and ADG requirements Issue Date Print Date SGHSAUSENRISK SECTION 1 IDENTIFICATION OF THE SUBSTANCE / MIXTURE AND OF THE COMPANY / UNDERTAKING Product Identifier Product name GUANIDINE HYDROCHLORIDE Chemical Name guanidine hydrochloride SynonymsSafety Data Sheet (SDS) Page 1 of 8 Date of Issue 6M Guanidine Hydrochloride Solution Section 1 Identification Product Identifier Catalog Number G0316 Recommended use of the chemical and restrictions on use For research use only Not intended for human or animal diagnostic or therapeutic uses
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Material Safety Data Sheet Guanidine Hydrochloride, 98% ACC# Section 1 Chemical Product and Company Identification MSDS Name Guanidine Hydrochloride, 98% Catalog Numbers AC, AC, AC, AC, AC, AC AC, AC, AC, ACE7492 FAQ Is the rate of TEV Protease cleavage affected by urea, guanidine hydrochloride and/or SDS? The guanidine is likely precipitating SDS, which is not terribly soluble in many highionic strength solutions Even if it was, the high ionic strength of 6M guanide would be problematic for
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SAFETY DATA SHEET Creation Date 16Nov10 Revision Date 18Jan18 Revision Number 3 1 Identification Product Name Guanidine hydrochloride Cat No FSHO CASNo Synonyms Aminoformamidine hydrochloride;Buy high purity Guanidine hydrochloride analytical standards from Chem Service Inc at $1770 () (NG)Guanidine hydrochloride >95 4 Firstaid measures General Advice If symptoms persist, call a physician Eye Contact Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes Get medical attention Skin Contact Wash off immediately with plenty of water for at least 15 minutes Obtain medical attention
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Guanidine HCl at millimolar concentrations, is able to causes efficient loss of the normally stable PSI element from yeast cells 5 mM Guanidine HCl in growth media cures PSI and other prions of yeast 5 mM Guanidine HCl significantly reduces Hsp104mediated basal and acquired thermotolerance by 30fold and 50 fold, respectivelyGuanidine hydrochloride For Molecular Biology CASNo ECNo IndexNo 4 First Aid Measures 41 Description of first aid measures General advice Consult a physician Show this safety data sheet to the doctor in attendance If inhaled If breathed in, move person into fresh airIf not breathing, give artificialFor example, protein samples containing 6M guanidineHCl will precipitate when mixed with Laemmli buffer for SDSPAGE, causing the sample to run poorly on a gel Fortunately, samples containing a wide range of interfering chemicals, such as chaotropic agents, detergents, lipids, pH extremes and salts, can be cleaned up within minutes using the SDSPAGE Sample Prep Kit
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Description In deionized water buffered with sodium hydroxide solution and TRIS hydrochlorideApplications Guanidine hydrochloride is used for purification of proteins and nucleic acids It can be used as a medicine, organic synthesis intermediate and is used in dyes It acts as an intermediate in the preparation of sulfadiazine, which is an important raw material for sulfamethyldiazine, sulfamethazine and folic acidThiocyanic acid, compound with guanidine (11) >95 4 Firstaid measures General Advice Show this safety data sheet to the doctor in attendance Immediate medical attention is required Eye Contact Rinse immediately with plenty of
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Guanidine HCl (Aminoformamidine), the crystalline compound of strong alkalinity formed by the oxidation of guanine, is a normal product of protein metabolism and a protein denaturant In vitro Guanidine HCl is the most popular protein denaturant Analysis of unfolding transitions by Guanidine HCl provides several important parameters regardingFind SigmaAldrich MSDS, related peerreviewed papers, technical documents, similar products & more at SigmaAldrichGUANIDINE HYDROCHLORIDE Safety Data Sheet according to Federal Register / Vol 77, No 58 / Monday, / Rules and Regulations EN (English US) 2/7 Name Product identifier % Classification (GHSUS) GUANIDINE HYDROCHLORIDE (Main constituent) (CAS No) 100 Acute Tox 4 (Oral), H302 Skin Irrit 2, H315
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Guanidine Hydrochloride Safety Data Sheet (SDS) SDS # 1017 Revision Date SECTION 1 — CHEMICAL PRODUCT AND COMPANY IDENTIFICATION Guanidine Hydrochloride Flinn Scientific, Inc PO Box 219, Batavia, IL (800) Chemtrec Emergency Phone Number (800)Safety Data Sheet (SDS) Page 2 of 10 Date of Issue 6 M Guanidine Thiocyanate Buffer Section 3 Composition/Information On Ingredients Chemical Name Identifiers Hazardous Approximate Percentage % Guanidine Thiocyanate CAS hazardous 70% TrisHCl CAS nonhazardous % Water CAS N/A nonhazardousMSDS Name Guanidine hydrochloride Catalog Numbers AC, AC, AC, AC, AC, AC, AC, AC
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Linear Formula NH2C(=NH)NH2 HCl;GuanidineHCl, Molecular Biology Grade (Guanidinium Hydrochloride) 100g $ 40 Your price Log in Change Configuration GuanidineHCl, Molecular Grade, is commonly used for the isolation of intact mRNA from tissues or cultured cells Formula Weight 9553 Form Fine,Guanidine hydrochloride (GdnHCl)1 is often used in protein purification procedures for the solubilization of proteins aggregated in inclusion bodies or
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GUANIDINE HYDROCHLORIDE CAS No MATERIAL SAFETY DATA SHEET SDS/MSDS SECTION1 Identificationof the substance/mixture and of the company/undertaking 11 Product identifiers Product name Guanidine Hydrochloride CASNo 12 Relevant identified uses of the substanceor mixtureand uses advised againstGuanidine hydrochloride 50 01 1 >= 30 < 50 maleic acid 7 >= 01 < 1 SECTION 4 FIRST AID MEASURES General advice Move out of dangerous area Show this material safety data sheet to the doctor in attendance If inhaled If unconscious place in recovery position and seek medical advice If symptoms persist, call a physician30 M potassium acetate, pH 55
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Guanidine hydrochloride (Grade I) and dithiothreitol (DTT) were from the Sigma Chemical Co 35Ssulfate at >1000 Ci/mmol was from New England Nuclear Absolute ethanol (dehydrated 0 proof) was from Pharmco Products, Inc BioGel A 05 M chromatography resin and polyacrylamide gel electrophoresis reagents were from BioRadUsing Table A3A1 as a guideline for quantities, guanidine·HCl and water (or buffer) can be weighed in a container to give the approximate molarities indicated A table of the refractive indexes of guanidine·HCl solutions from 0057 M to 851 M (in increments of ~006 M) at 25°C is provided by Nozaki (1972)Material Safety Data Sheet or SDS for Guanidine Hydrochloride, Molecular Biology Grade CAS Calbiochem from MilliporeSigma for download or viewing in the browser Catalog Number Product Name Guanidine Hydrochloride, Molecular Biology Grade
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Guanidine hydrochloride (GuHCl) is a small hydroscopic molecule It plays a role in inhibiting heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) activity in vivo It is a potent denaturant and inactivator of several enzymes and proteinsGuanidinium chloride LAB CAS , EC Number , chemical formula H₂NC(NH)NH₂ * HCl Find MSDS or SDS, a COA, data sheets and more informationThese kits contain guanidine salts (eg, guanidine thiocyanate and guanidine hydrochloride) that may produce hazardous gases when combined with
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4 M guanidine isothiocyanate, 50 mM TrisHCl (pH 75), 25 mM EDTA Cat No Size 500 ml Store at 4°C Description Guanidine isothiocyanate is a strong protein denaturant In a highly concentrated guanidine isothiocyanate solution, many proteins unfold and separate into their constituent polypeptides Guanidine Isothiocyanate isP2 (lysis buffer) (QIAGEN cat# , 500ml) 0 mM NaOH, 1% SDS N3 (neutralization buffer for DNA binding) (QIAGEN cat# , 500ml) 42 M guanidine hydrochloride (GuHCl), 09 M potassium acetate, pH 48 P3 (neutralization buffer for midi, maxi, giga tips) DO NOT USE for spin columns, use N3;Guanidine Hydrochloride, Molecular Biology Grade CAS Calbiochem Powerful protein denaturant Find MSDS or SDS, a COA, data sheets and more information
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Guanidine Hydrochloride Product Code(s) Revision Date 08Aug11 MSDS M110 Target Organ Effects Bone marrow, Nerves Product Information Harmful if swallowed, inhaled, or absorbed through skin Causes skin, eye and respiratory tract irritation 10 STABILITY AND REACTIVITY Hazardous Polymerization Hazardous polymerization does not occurGUANIDINE HCL, 80M SOLUTION Safety Data Sheet according to Federal Register / Vol 77, No 58 / Monday, / Rules and Regulations EN (English US) 2/6 Name Product identifier % Classification (GHSUS) guanidine,hydrochloride (CAS No) 70 80 Acute Tox 4 (Oral), H302 Skin Irrit 2, H315Thermo Scientific Pierce 8M GuanidineHCl Solution is an accurately formulated, readytouse solution of guanidine hydrochloride, that can be easily diluted and pHadjusted to any concentration below 8M Features of 8M GuanidineHCl Solution • Any concentration below 8M guanidine can be quickly and easily prepared
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